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expression plasmid pet30a  (Promega)

 
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    Promega expression plasmid pet30a
    Construction of <t>pET30a</t> -HSP70 vectors and recombinant protein expression and purification. (A) Schematic representation of the recombinant pET30a- HSP70 vectors. The full-length HSP70 gene was cloned into the vector pET30a using the restriction sites EcoR1 and Hind III. (B) RT-PCR confirmation of the recombinant HSP70 . Lane 1: the full-length HSP70 . Lane 2: double enzyme-digested 2,000-bp gene fragment. (C) HSP70 protein expressed by recombinant vector pET30 -HSP70 . Lane M: protein molecular weight markers. Lanes 1–2: whole cell lysate of E. coli Rosetta 2 transformed with pET30- HSP70 without [1] and with [2] induction of IPTG. Lane 3: purified HSP70 protein at 1× loading concentration. HSP70, heat shock protein 70; RT-PCR, reverse transcription-polymerase chain reaction; IPTG, isopropyl β-d-thiogalactoside.
    Expression Plasmid Pet30a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression plasmid pet30a/product/Promega
    Average 90 stars, based on 1 article reviews
    expression plasmid pet30a - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Fusion of NY-ESO-1 epitope with heat shock protein 70 enhances its induced immune responses and antitumor activity against glioma in vitro"

    Article Title: Fusion of NY-ESO-1 epitope with heat shock protein 70 enhances its induced immune responses and antitumor activity against glioma in vitro

    Journal: Translational Cancer Research

    doi: 10.21037/tcr-23-1476

    Construction of pET30a -HSP70 vectors and recombinant protein expression and purification. (A) Schematic representation of the recombinant pET30a- HSP70 vectors. The full-length HSP70 gene was cloned into the vector pET30a using the restriction sites EcoR1 and Hind III. (B) RT-PCR confirmation of the recombinant HSP70 . Lane 1: the full-length HSP70 . Lane 2: double enzyme-digested 2,000-bp gene fragment. (C) HSP70 protein expressed by recombinant vector pET30 -HSP70 . Lane M: protein molecular weight markers. Lanes 1–2: whole cell lysate of E. coli Rosetta 2 transformed with pET30- HSP70 without [1] and with [2] induction of IPTG. Lane 3: purified HSP70 protein at 1× loading concentration. HSP70, heat shock protein 70; RT-PCR, reverse transcription-polymerase chain reaction; IPTG, isopropyl β-d-thiogalactoside.
    Figure Legend Snippet: Construction of pET30a -HSP70 vectors and recombinant protein expression and purification. (A) Schematic representation of the recombinant pET30a- HSP70 vectors. The full-length HSP70 gene was cloned into the vector pET30a using the restriction sites EcoR1 and Hind III. (B) RT-PCR confirmation of the recombinant HSP70 . Lane 1: the full-length HSP70 . Lane 2: double enzyme-digested 2,000-bp gene fragment. (C) HSP70 protein expressed by recombinant vector pET30 -HSP70 . Lane M: protein molecular weight markers. Lanes 1–2: whole cell lysate of E. coli Rosetta 2 transformed with pET30- HSP70 without [1] and with [2] induction of IPTG. Lane 3: purified HSP70 protein at 1× loading concentration. HSP70, heat shock protein 70; RT-PCR, reverse transcription-polymerase chain reaction; IPTG, isopropyl β-d-thiogalactoside.

    Techniques Used: Recombinant, Expressing, Purification, Clone Assay, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Transformation Assay, Concentration Assay, Reverse Transcription, Polymerase Chain Reaction



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    Construction of <t>pET30a</t> -HSP70 vectors and recombinant protein expression and purification. (A) Schematic representation of the recombinant pET30a- HSP70 vectors. The full-length HSP70 gene was cloned into the vector pET30a using the restriction sites EcoR1 and Hind III. (B) RT-PCR confirmation of the recombinant HSP70 . Lane 1: the full-length HSP70 . Lane 2: double enzyme-digested 2,000-bp gene fragment. (C) HSP70 protein expressed by recombinant vector pET30 -HSP70 . Lane M: protein molecular weight markers. Lanes 1–2: whole cell lysate of E. coli Rosetta 2 transformed with pET30- HSP70 without [1] and with [2] induction of IPTG. Lane 3: purified HSP70 protein at 1× loading concentration. HSP70, heat shock protein 70; RT-PCR, reverse transcription-polymerase chain reaction; IPTG, isopropyl β-d-thiogalactoside.
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    Image Search Results


    Construction of pET30a -HSP70 vectors and recombinant protein expression and purification. (A) Schematic representation of the recombinant pET30a- HSP70 vectors. The full-length HSP70 gene was cloned into the vector pET30a using the restriction sites EcoR1 and Hind III. (B) RT-PCR confirmation of the recombinant HSP70 . Lane 1: the full-length HSP70 . Lane 2: double enzyme-digested 2,000-bp gene fragment. (C) HSP70 protein expressed by recombinant vector pET30 -HSP70 . Lane M: protein molecular weight markers. Lanes 1–2: whole cell lysate of E. coli Rosetta 2 transformed with pET30- HSP70 without [1] and with [2] induction of IPTG. Lane 3: purified HSP70 protein at 1× loading concentration. HSP70, heat shock protein 70; RT-PCR, reverse transcription-polymerase chain reaction; IPTG, isopropyl β-d-thiogalactoside.

    Journal: Translational Cancer Research

    Article Title: Fusion of NY-ESO-1 epitope with heat shock protein 70 enhances its induced immune responses and antitumor activity against glioma in vitro

    doi: 10.21037/tcr-23-1476

    Figure Lengend Snippet: Construction of pET30a -HSP70 vectors and recombinant protein expression and purification. (A) Schematic representation of the recombinant pET30a- HSP70 vectors. The full-length HSP70 gene was cloned into the vector pET30a using the restriction sites EcoR1 and Hind III. (B) RT-PCR confirmation of the recombinant HSP70 . Lane 1: the full-length HSP70 . Lane 2: double enzyme-digested 2,000-bp gene fragment. (C) HSP70 protein expressed by recombinant vector pET30 -HSP70 . Lane M: protein molecular weight markers. Lanes 1–2: whole cell lysate of E. coli Rosetta 2 transformed with pET30- HSP70 without [1] and with [2] induction of IPTG. Lane 3: purified HSP70 protein at 1× loading concentration. HSP70, heat shock protein 70; RT-PCR, reverse transcription-polymerase chain reaction; IPTG, isopropyl β-d-thiogalactoside.

    Article Snippet: Prokaryotic expression plasmid pET30a (Promega, Madison, WI, USA) was kept in our laboratory.

    Techniques: Recombinant, Expressing, Purification, Clone Assay, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Transformation Assay, Concentration Assay, Reverse Transcription, Polymerase Chain Reaction